UK: Diageo net sales increased 9% to GBP7.260 million for the year ended 30 June 2006

Diageo released August 31 its Preliminary results for the year ended 30 June 2006.

Paul Walsh, Chief Executive of Diageo, commenting on the year ... More Info

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Estonia: Saku Olletehase sales increased 7.6% in 1H 2006

In the first half of 2006, Saku Olletehase AS sold over 42 million litres of beverages, a notable 7.6 per cent or 3 million ... More Info

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Czech Republic: Beer output increased 5% in the seven months of 2006

Czech breweries raised beer output by 5 percent year-on-year in the first seven months of the year owing to a hot July, said Jan ... More Info

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Denmark: Royal Unibrew A/S reports 6 month operating profit of DKK60.6 million

The Danish brewery group Royal Unibrew A/S reported 29 August an operating profit of DKK60.6 million on net revenue of DKK1.62 billion for the ... More Info

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USA: Heineken is in a light-beer war with itself as Amstel is eclipsed by Heineken Premium Light

Heineken's Amstel Light is struggling to maintain relevance within the import-light-beer category it invented, and the culprit may be ... Heineken Premium Light, Advertising ... More Info

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Australia: Foster's Group Ltd. denies takeover approach

Foster's Group Ltd., Australia's biggest beer and wine maker, said August 29 it was not aware of any takeover proposals, sending its shares down ... More Info

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Czech Republic: Malt production increased 2% year-on-year for 1H 2006

According to the Czech Beer and Malt Association statement , Czech malt houses raised output in the first half of 2006 by 2 percent ... More Info

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Belarus: Exports of malting barley recorded 38,500 t as of August 29

The Belarusian Ministry of Agriculture and Food (MAF) controls the sales of malting barley on account of government buying. By August 29 the enterprises ... More Info

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Australia: Barley prices re-surge as fear of poor harvest intensifies

Old and new-crop barley prices are surging as fears of a poor harvest grow. Dry weather is expected to cut the national crop from ... More Info

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USA: Hops were under less stress in USA than in Europe

The latter half of July brought with it above average temperatures on both sides of the Atlantic. In the United States, this slowed ... More Info

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ABSTRACT
Feed and malting barley are major crops in Montana. Harrington, a two-rowed spring malting barley variety, is the most cultivated variety in the state. Barley was the last of the world’s major cereals for which transformation methods were developed because in vitro-cultured barley rapidly loses regeneration ability or gives rise to albino plants during selection for transformed tissue. Previous research used the variety Golden Promise because it regenerates well under research conditions, though it is not commercially used. Transformation could be an important method to improve varieties in North American barley cultivars. The puroindoline proteins (PINA and PINB) can be isolated from wheat endosperm and are basic cysteine and tryptophan-rich proteins that might play a role in defense against pathogens. The puroindolines show antifungal activity both in vitro and in vivo. Milk stage seeds were harvested to obtain embryos. Embryos were placed on induction media [2.5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.01 mg/L 6-benzylaminopurine (BAP)] for 12 to 51days until calli formed. The biolistic method was chosen for transmitting the gene of interest into the calli, using a Biolistic PDS-1000/He Particle Delivery System (BioRad, Hercules, CA) with 900 psi rupture disks. Approximately 4,500 calli were bombarded with two plasmids; pinA driven by the maize ubiquitin promoter (Ubi1) which is expressed constitutively, and the hygromycin phosphotransferase (hph), which confers hygromycin B resistance and is driven by the CaMV 35S promoter for selection. The bombarded calli were selected on medium containing 30 mg/L Hygromycin B for 9 to 21 days, and subcultured to intermediate medium (1.0mg/L 2,4-D and 0.5 mg/L BAP), followed by regeneration media (1.0-3.0 mg/L BAP). Thirty hygromycin B resistant calli regenerated and 5 died in magenta box. Thirty five putative transgenic plants derived from 25 calli grew in soil and were harvested. Some plants tested by polymerase chain reaction (PCR) tested positive with primers for pinA and hph. Southern blots were performed to detect the presence of hph in T1 barley genomic DNA but were negative. Northern blots performed to detect the presence of pinA and hph transcribed RNA in the T1leaf tissue were also negative, showing that no stably transformed plants were obtained.
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